Identification of capsid mutations that alter the rate of HIV - 1 uncoating 3 in infected cells
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330 19 Text5693 20 21 JVI Accepts, published online ahead of print on 22 October 2014 J. Virol. doi:10.1128/JVI.03043-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved. on A uust 5, 2017 by gest http/jvi.asm .rg/ D ow nladed fom
منابع مشابه
Identification of capsid mutations that alter the rate of HIV-1 uncoating in infected cells.
UNLABELLED After viral fusion with the cell membrane, the conical capsid of HIV-1 disassembles by a process called uncoating. We recently utilized the cyclosporine (CsA) washout assay, in which TRIM-CypA-mediated restriction of viral replication is used to detect the state of the viral capsid, to study the kinetics of uncoating in HIV-1-infected cells. Here we have extended this analysis to exa...
متن کاملContribution of PDZD8 to Stabilization of the Human Immunodeficiency Virus (HIV-1) Capsid
Following human immunodeficiency virus (HIV-1) entry into the host cell, the viral capsid gradually disassembles in a process called uncoating. A proper rate of uncoating is important for reverse transcription of the HIV-1 genome. Host restriction factors such as TRIM5α and TRIMCyp bind retroviral capsids and cause premature disassembly, leading to blocks in reverse transcription. Other host fa...
متن کاملEarly cytoplasmic uncoating is associated with infectivity of HIV-1.
After fusion, HIV delivers its conical capsid into the cytoplasm. To release the contained reverse-transcribing viral genome, the capsid must disassemble in a process termed uncoating. Defining the kinetics, dynamics, and cellular location of uncoating of virions leading to infection has been confounded by defective, noninfectious particles and the stochastic minefield blocking access to host D...
متن کاملCytoplasmic Dynein Promotes HIV-1 Uncoating
Retroviral capsid (CA) cores undergo uncoating during their retrograde transport (toward the nucleus), and/or after reaching the nuclear membrane. However, whether HIV-1 CA core uncoating is dependent upon its transport is not understood. There is some evidence that HIV-1 cores retrograde transport involves cytoplasmic dynein complexes translocating on microtubules. Here we investigate the role...
متن کاملEvidence for a functional link between uncoating of the human immunodeficiency virus type 1 core and nuclear import of the viral preintegration complex.
Human immunodeficiency virus type 1 (HIV-1) particles begin their replication upon fusion with the plasma membrane of target cells and release of the viral core into the host cell cytoplasm. Soon thereafter, the viral capsid, which is composed of a polymer of the CA protein, disassociates from the internal ribonucleoprotein complex. While this disassembly process remains poorly understood, the ...
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تاریخ انتشار 2014